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second strand cdna synthesis cocktail  (Thermo Fisher)


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    Structured Review

    Thermo Fisher second strand cdna synthesis cocktail
    Second Strand Cdna Synthesis Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+strand+cdna+synthesis+cocktail/TRIS-HCL/pm16608287__nl0603350si20060213_083213-64-82-89
    Average 99 stars, based on 1 article reviews
    second strand cdna synthesis cocktail - by Bioz Stars, 2026-09
    99/100 stars

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    cDNA Synthesis:

    Article Title:
    Article Snippet: .. 10μl of First Strand cDNA Synthesis cocktail (4μl of Affymetrix 5X 1st strand buffer (250 mM Tris-HCl, pH 8.3 at room temperature; 375 mM KCl; 15 mM MgCl2), is mixed with 2μl 0.1M DTT, 1μl 10mM dNTP mix, 1μl Superscript II (200U/ul), and 2μl nuclease free water per reaction) is added, and the plate is then transferred to the thermal cycler and incubated at 420C for 60 minutes and 40C for 5 min. 91μl of nuclease free water and 39μl of the Second Strand cDNA Synthesis cocktail (30μl of Affymetrix 5X 2nd strand buffer, 100mM Tris-HCl (pH 6.9), 23mM MgCl2, 450mM KCl, 0.75mM ß-NAD, 50mM (NH4)2SO4); 3μl 10mM dNTP; 1μl 10unit/μl DNA Ligase; 4μl 10unit/μl DNA Polymerase and 1μl 2units/μl RNase H) is added. ..

    Incubation:

    Article Title:
    Article Snippet: .. 10μl of First Strand cDNA Synthesis cocktail (4μl of Affymetrix 5X 1st strand buffer (250 mM Tris-HCl, pH 8.3 at room temperature; 375 mM KCl; 15 mM MgCl2), is mixed with 2μl 0.1M DTT, 1μl 10mM dNTP mix, 1μl Superscript II (200U/ul), and 2μl nuclease free water per reaction) is added, and the plate is then transferred to the thermal cycler and incubated at 420C for 60 minutes and 40C for 5 min. 91μl of nuclease free water and 39μl of the Second Strand cDNA Synthesis cocktail (30μl of Affymetrix 5X 2nd strand buffer, 100mM Tris-HCl (pH 6.9), 23mM MgCl2, 450mM KCl, 0.75mM ß-NAD, 50mM (NH4)2SO4); 3μl 10mM dNTP; 1μl 10unit/μl DNA Ligase; 4μl 10unit/μl DNA Polymerase and 1μl 2units/μl RNase H) is added. ..



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    second strand cdna synthesis cocktail  (Thermo Fisher)
    99
    Thermo Fisher second strand cdna synthesis cocktail
    Second Strand Cdna Synthesis Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+strand+cdna+synthesis+cocktail/TRIS-HCL/pm16608287__nl0603350si20060213_083213-64-82-89
    Average 99 stars, based on 1 article reviews
    second strand cdna synthesis cocktail - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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